masson trichrome stain kit  (StatLab Medical Products Inc)

Journal: Signal Transduction and Targeted Therapy

Article Title: MAPK14 converges on key transcriptional machinery to promote vascular smooth muscle cell degeneration in abdominal aortic aneurysm

doi: 10.1038/s41392-025-02540-0

Figure Lengend Snippet: Deficiency of MAPK14 in VSMCs impedes Ang II-induced aortic remodeling and VSMC degeneration. Mapk14 WT ( Mapk14 f/f / Apoe -/- ) and Mapk14 KO ( Sm22- Cre +/- / Mapk14 f/f /Apoe -/- ) mice were infused with Angiotensin II (Ang II) for 7 days, and abdominal aortas (AAs) were isolated for the following experiments. a Representative images of hematoxylin and eosin (H&E), Masson’s trichrome, and Picrosirius Red staining of cross sections of AAs from the indicated mice. b Quantification of vessel wall thickness (medial and adventitial layers) via H&E staining ( n = 6 per group). c Quantification of fibrosis via Masson’s trichrome and Picrosirius Red staining ( n = 6 per group). d Representative immunofluorescence images of COL1 (left) and dihydroethidium (DHE) staining (right) in AAs from the indicated mice. e , f Quantification of the COL1-positive area ( e ) and DHE positive area ( f ) in AAs ( n = 6 per group). g Representative Western blot images of the indicated proteins in AAs from the indicated mice. h Quantification of the levels of the indicated proteins in the indicated groups ( n = 7 per group for HSPA5, PBH4, ATF4, and cl-CASP3; n = 4 per group for ATF6). i Representative immunofluorescence images showing PBH4, HSPA5, cl-CASP3 (green), ACTA2 (red), and DAPI (blue) in cross-sections of AAs. j Quantification of the positive area of the indicated proteins within the media layer of AAs ( n = 4 per group). k Representative transmission electron microscopy (TEM) images showing swollen mitochondria and enlarged endoplasmic reticulum (ER) in Mapk14 WT compared with Mapk14 KO mice ( n = 3 per group). Red arrows indicate swollen mitochondria, and white arrows indicate enlarged ER. The data were analyzed via Student’s t tests for all quantifications. L lumen; M medial layer

Article Snippet: Paraffin-embedded AA cross sections (5 μm) were deparaffinized, rehydrated, and then subjected to standard hematoxylin and eosin (H&E), Masson’s trichrome (StatLab, KTMTR2), or Picrosirius Red (Abcam, ab150681) staining for morphometric analysis.

Techniques: Isolation, Staining, Immunofluorescence, Western Blot, Transmission Assay, Electron Microscopy